![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4897013:Design
Designed by: Hejia Dong Group: iGEM23_BS-United-China (2023-10-06)
Dark suicide system based on Cas9
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 5114
Illegal NheI site found at 5137
Illegal NheI site found at 5296
Illegal NheI site found at 5319
Illegal NheI site found at 5478
Illegal NheI site found at 5501 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 702
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 256
Illegal AgeI site found at 366 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Notice the dark environment will activate the suicide system and kill the E. coli
Source
EL222 and Cas9 gene are from bacterail