Composite

Part:BBa_K4897013:Design

Designed by: Hejia Dong   Group: iGEM23_BS-United-China   (2023-10-06)


Dark suicide system based on Cas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5114
    Illegal NheI site found at 5137
    Illegal NheI site found at 5296
    Illegal NheI site found at 5319
    Illegal NheI site found at 5478
    Illegal NheI site found at 5501
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 702
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 256
    Illegal AgeI site found at 366
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Notice the dark environment will activate the suicide system and kill the E. coli


Source

EL222 and Cas9 gene are from bacterail

References